9MRS
Crystal structure of human MMACHC in complex with MMADHC and B12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-10-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.764, 67.983, 192.660 |
| Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
| Resolution | 41.245 - 3.400 |
| R-factor | 0.3 |
| Rwork | 0.300 |
| R-free | 0.35740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.457 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.013 | 3.700 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 13788 | 689 |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 81.0 | |
| Redundancy | 3 | |
| CC(1/2) | 0.974 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 0.1M Bis-Tris, pH 6.5, and 0.325M ammonium sulphate |
