9MR6
X-ray crystal structure of SAMHD1 from Rhizophagus irregularis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979354 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.359, 85.420, 87.035 |
| Unit cell angles | 104.58, 111.65, 99.51 |
Refinement procedure
| Resolution | 41.500 - 2.270 |
| R-factor | 0.2233 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.040 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.102 | 0.877 |
| Rmeas | 0.144 | 1.239 |
| Rpim | 0.102 | 0.875 |
| Total number of observations | 22472 | |
| Number of reflections | 91728 | 13106 |
| <I/σ(I)> | 2.8 | 0.6 |
| Completeness [%] | 94.6 | |
| Redundancy | 1.8 | 1.7 |
| CC(1/2) | 0.991 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M ammonium tartrate, 20% (w/v) PEG 3350 |
