9MMX

M6 protein with Factor H 6-7 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	33.483, 69.546, 293.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.900 - 1.900
R-factor	0.2624
Rwork	0.260
R-free	0.30210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.688
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	55717	3542
<I/σ(I)>	9.8
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	0.999	0.304

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 6000, ethylene glycol, Sodium chloride