9MLF

TUBULIN-RB3_SLD IN COMPLEX WITH COMPOUND QW-4-183-llb

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92012
Spacegroup name	P 21 21 21
Unit cell lengths	66.482, 126.974, 252.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 2.400
R-factor	0.2002
Rwork	0.199
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.350	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	84508	4420
<I/σ(I)>	10.6
Completeness [%]	100.0	100
Redundancy	8.5	8.5
CC(1/2)	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1 M trisodium citrate, pH 5.6, 0.2 M ammonium sulfate, 12.2% PEG 4000