9ML8
Crystal structure of the SARS-CoV-2 RBD in complex with the rabbit M8b-B1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.074, 173.441, 129.928 |
| Unit cell angles | 90.00, 116.55, 90.00 |
Refinement procedure
| Resolution | 39.150 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.150 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.172 | 0.989 |
| Rmeas | 0.185 | 1.063 |
| Rpim | 0.068 | 0.388 |
| Number of reflections | 133293 | 6593 |
| <I/σ(I)> | 7.3 | 2.3 |
| Completeness [%] | 98.5 | 99.1 |
| Redundancy | 7.2 | 7.4 |
| CC(1/2) | 0.991 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate 0.1 sodium citrate 20% (w/v) PEG 1,000 |
