9MK7

Crystal structure of the WDR domain of WDR91 in complex with DR3634

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2024-10-08
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	79.201, 132.236, 118.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.900 - 2.310
R-factor	0.20171
Rwork	0.200
R-free	0.23777
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.405
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.310	6.270	2.310
Rmerge	0.071	0.024	0.971
Rmeas	0.076	0.025	1.049
Rpim	0.027	0.009	0.390
Total number of observations	215906
Number of reflections	27368	1460	1343
<I/σ(I)>	12.5
Completeness [%]	99.9	98.1	100
Redundancy	7.9	7.3	7
CC(1/2)	0.999	1.000	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG 8000, 20% v/v ethylene glycol, 0.02M of each alcohol,0.1M MES/imidazole pH6.5