9MI6
Crystal structure of human FcRn in complex with nipocalimab Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.282, 67.644, 105.303 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 44.480 - 2.410 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.736 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.480 | 2.496 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.144 | 0.956 |
| Rpim | 0.095 | 0.630 |
| Number of reflections | 46599 | 4604 |
| <I/σ(I)> | 4.78 | 1.02 |
| Completeness [%] | 95.9 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 10% PEG 1000, 10% PEG 8000 |
