9MF0

Crystal structure of KRAS(GDP) bound to LZTR1(Kelch domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97627
Spacegroup name	P 21 21 2
Unit cell lengths	129.736, 138.300, 199.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.210 - 3.300
R-factor	0.1884
Rwork	0.186
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.040	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.332	1.647
Rpim	0.148	0.720
Number of reflections	54824	4446
<I/σ(I)>	6.6
Completeness [%]	100.0
Redundancy	11.1
CC(1/2)	0.990	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	8% w/v PGA-LM. 0.3M sodium malonate dibasic monohydrate, 0.1M sodium acetate pH 5.0.