9MC0

The co-crystal structure of DYRK2 with AF-4

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2023-10-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	129.850, 60.550, 155.280
Unit cell angles	90.00, 113.11, 90.00

Refinement procedure

Resolution	58.480 - 2.870
R-factor	0.2478
Rwork	0.244
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.429
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.480	2.940
High resolution limit [Å]	2.870	2.870
Rmerge	0.122	1.017
Number of reflections	25668	1775
<I/σ(I)>	9.9
Completeness [%]	99.7	99.83
Redundancy	6.7
CC(1/2)	0.996	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.05M HEPES pH7.0 15% PEG3350 1% Trptone 1mM NaN3