9M41
The crystal structure of full-length PAK2 containing K278R and D368N mutants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.824, 55.844, 146.407 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.020 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (v2.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.162 | 0.825 |
| Number of reflections | 17583 | 1786 |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 95.6 | 99.2 |
| Redundancy | 2.7 | 2.8 |
| CC(1/2) | 0.969 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 21% PEG 3350, 0.1M Na/K phosphate pH 6.1 |
