9M0O

Crystal structure of OPTN 138-170 in complex with GTP-bound RAB8A1-176 (Q67L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	70.712, 70.712, 109.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 1.830
R-factor	0.1911
Rwork	0.190
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.830	1.830
Rmeas	0.242
Number of reflections	25176	25125
<I/σ(I)>	18.46
Completeness [%]	100.0
Redundancy	25.9
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.05 M Imidazole pH 6.5, 0.5 M Sodium acetate trihydrate