9M0H
Crystal structure of human endonuclease G mutant C113A/H141A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.992, 73.745, 89.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.630 - 1.850 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6njt |
| RMSD bond angle | 6.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.010 | 1.850 |
| Rmerge | 0.053 | 0.020 | 0.574 |
| Total number of observations | 272549 | ||
| Number of reflections | 39047 | 2005 | 1920 |
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 99.6 | 93.9 | 100 |
| Redundancy | 7 | 5.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.02 M Citric acid, 0.08 M BIS-TRIS propane pH 8.0, 16% w/v Polyethylene glycol 3350 |
