9LUX

Single-chain Fv antibody of G2 fused with antigen peptide from chicken prion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	86.360, 86.720, 86.580
Unit cell angles	105.13, 107.91, 100.88

Refinement procedure

Resolution	49.320 - 1.850
R-factor	0.1959
Rwork	0.194
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pgx
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.850	1.850
Rmerge	0.094	0.786
Number of reflections	183582	29434
<I/σ(I)>	8.5	1.8
Completeness [%]	97.3
Redundancy	3.2	3.2
CC(1/2)		0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M ammonium sulphate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 1.0 M lithium sulfate monohydrate