9LUW

Enhancing Monodispersity and Thermal Stability of Human H-Ferritin for Improved Applications in Nanocarrier Systems

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9
Spacegroup name	P 2 3
Unit cell lengths	180.592, 180.592, 180.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 2.000
R-factor	0.1794
Rwork	0.179
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.854
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.690	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	131611	131611
<I/σ(I)>	14.40625
Completeness [%]	100.0
Redundancy	19.2
CC(1/2)	0.989	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	sodium phosphate monobasic , potassium phosphate dibasic, imidazole, NaCl