9LUK
Crystal structure of Switchbody based on anti-osteocalcin antibody KTM219 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.841, 65.909, 69.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.920 - 1.950 |
| R-factor | 0.20379 |
| Rwork | 0.202 |
| R-free | 0.23753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x5x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.465 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 1.256 |
| Rmeas | 0.076 | 1.308 |
| Rpim | 0.021 | 0.364 |
| Total number of observations | 431822 | |
| Number of reflections | 32857 | 2290 |
| <I/σ(I)> | 23.9 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 12.7 |
| CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M Bis-tris, 22% Polyethylene glycol monomethyl ether 2000 |
