9LTG

Crystal structure of H-2Kb with C.parvum peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2023-10-18
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	66.552, 79.898, 97.958
Unit cell angles	78.00, 71.24, 76.28

Refinement procedure

Resolution	91.798 - 2.530
Rwork	0.228
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.800	2.600
High resolution limit [Å]	2.530	2.530
Rmerge		0.054
Rpim		0.054
Number of reflections	55839	55838
<I/σ(I)>	10.8
Completeness [%]	96.0
Redundancy	1.8
CC(1/2)	0.998	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.2M Sodium formate(PH7.0), 20% (w/v) Polyethylene glycol 3350