9LSM

The crystal structure of PDE5A with L9

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-24
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	73.944, 73.944, 131.556
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.340 - 2.700
R-factor	0.2555
Rwork	0.253
R-free	0.30410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h42
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	CrysalisPro (v2)
Refinement software	REFMAC (V1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.340	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.260	0.930
Number of reflections	11922	1175
<I/σ(I)>	7
Completeness [%]	99.9	100
Redundancy	8.5	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M MgSO4, 0.1 M Cacodylate sodium salt, pH 6.5, 2.5% ethanol, and 18% PEG3350