Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.815, 83.815, 163.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.630 - 1.870 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.033 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.600 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.100 | 0.954 |
| Rmeas | 0.102 | 0.982 |
| Rpim | 0.020 | 0.228 |
| Total number of observations | 1052358 | 99076 |
| Number of reflections | 43669 | 5591 |
| <I/σ(I)> | 19.2 | 2.7 |
| Completeness [%] | 88.3 | |
| Redundancy | 24.1 | 17.7 |
| CC(1/2) | 0.999 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1 M potassium sodium tartrate, 100 mM imidazole, and 200 mM sodium chloride |
