9LNU

Structure of MBP tagged H2A.W-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	109.115, 109.115, 151.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.670 - 1.850
R-factor	0.2085
Rwork	0.208
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k0c
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.187	0.108	2.217
Rmeas	0.191	0.111	2.265
Rpim	0.038	0.023	0.461
Total number of observations	2007052
Number of reflections	79451	8392	7829
<I/σ(I)>	4.8
Completeness [%]	100.0	99.8	100
Redundancy	25.3	23.4	23.7
CC(1/2)	0.998	0.997	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Ammonium dihydrogen phosphate, 0.1 M Tris pH=7.5, 20% PEG 3350.