9LJW

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2024-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	142.606, 142.606, 91.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.680 - 3.130
Rwork	0.183
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.482
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.590	3.300
High resolution limit [Å]	3.130	3.130
Number of reflections	17165	2741
<I/σ(I)>	7.8	1.4
Completeness [%]	90.7
Redundancy	11.9
CC(1/2)	0.994	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant