9LDS

Crystal structure of nanobody H145 bound to SARS-CoV-2 spike stem-helix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	43.073, 63.539, 56.064
Unit cell angles	90.00, 112.75, 90.00

Refinement procedure

Resolution	40.100 - 1.600
R-factor	0.1917
Rwork	0.191
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.106	0.596
Number of reflections	36597	3629
<I/σ(I)>	22.519
Completeness [%]	100.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES pH 7.5, 70% v/v (+/-)-2-Methyl-2,4-pentanediol