9LB5

mutant S483T/G490N/V492M/S497T/A599G of HEV-1 E2s domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	100.209, 100.209, 62.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.815 - 1.350
R-factor	0.1491
Rwork	0.139
R-free	0.15410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.370
High resolution limit [Å]	1.350	3.660	1.350
Rmerge		0.065	0.483
Rmeas		0.068	0.500
Rpim	0.029	0.018	0.132
Number of reflections	136609	6984	6825
<I/σ(I)>	11.9
Completeness [%]	100.0	99.5	100
Redundancy	14.7	14.4	14.3
CC(1/2)		0.988	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.1 M NaAc and 2 M (NH4)2SO4