9L90
The crystal structure of human RyR3 Repeat12 domain in complex with Dantrolene and AMP-PCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2024-09-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.739, 60.839, 262.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 131.260 - 2.790 |
| Rwork | 0.272 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.260 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.068 | 0.821 |
| Rmeas | 0.061 | 0.851 |
| Rpim | 0.026 | 0.209 |
| Total number of observations | 304066 | 45462 |
| Number of reflections | 25177 | 3580 |
| <I/σ(I)> | 13.2 | 3.8 |
| Completeness [%] | 99.3 | |
| Redundancy | 6.5 | 6 |
| CC(1/2) | 0.993 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.7M sodium acetate trihydrate, 14% peg 3350 |
