9L8C
Structure of human TREX1-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-04-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.310, 92.787, 161.508 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 44.300 - 2.520 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tqq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rpim | 0.077 | 0.346 |
| Number of reflections | 43791 | 4177 |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 99.1 | 94.35 |
| Redundancy | 8.3 | 8.4 |
| CC(1/2) | 0.969 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M Li2SO4, 22.5% PEG-3350, 0.1M Bis-Tris pH 6.5 |
