9L6Y
Crystal structure of the L7Ae derivative protein LS12 in complex with its co-evolved target CS2 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.300, 55.280, 83.050 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 45.420 - 3.120 |
| R-factor | 0.2237 |
| Rwork | 0.220 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.756 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmeas | 0.170 | 1.128 |
| Number of reflections | 8762 | 1433 |
| <I/σ(I)> | 8.13 | 1.44 |
| Completeness [%] | 98.0 | |
| Redundancy | 4 | |
| CC(1/2) | 0.993 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% PEG300, 100 mM Sodium cacodylate/HCl, pH 6.5, 200 mM Calcium acetate |
