9L5Q

Crystal structure of PI5P4Kalpha N251S in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-02-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.9000
Spacegroup name	P 61 2 2
Unit cell lengths	136.106, 136.106, 94.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 2.500
R-factor	0.2034
Rwork	0.202
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.600
High resolution limit [Å]	2.500	2.500
Rpim	0.030	0.321
Number of reflections	18405	2025
<I/σ(I)>	23.2	2.5
Completeness [%]	99.8
Redundancy	39.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0M sodium citrate, 0.01M sodium borate pH 8.5