9L49

Crystal structure of HLA-C*12:03-RV9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	95
Detector technology	CCD
Collection date	2023-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	I 2 2 2
Unit cell lengths	85.309, 95.270, 119.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.650 - 3.000
R-factor	0.2067
Rwork	0.203
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.529
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.182	0.466
Rmeas	0.198	0.506
Rpim	0.078	0.195
Number of reflections	10021	1593
<I/σ(I)>	12.9
Completeness [%]	99.8	100
Redundancy	11.6	12.5
CC(1/2)	0.993	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.5 M Ammonium sulfate 0.1 M Sodium HEPES pH7.5 2% v/v PEG400