9L2W

Crystal structure of the C-terminal of viral responsive protein 15 (PmVRP15) from black tiger shrimp Penaeus monodon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2024-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	66.487, 115.309, 54.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.280 - 1.500
Rwork	0.160
R-free	0.17860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.317
Data reduction software	XDS (Jun 30, 2024)
Data scaling software	XDS (Jun 30, 2024)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.280	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.066	0.338
Rmeas	0.069	0.353
Rpim	0.019	0.098
Number of reflections	33500	3239
<I/σ(I)>	26.88	6.81
Completeness [%]	98.3	96.89
Redundancy	12.8	12.6
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium sulfate, sodium chloride, CHES