9L2Q

Crystal structure of anti-CRISPR protein AcrIE7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	108.714, 108.714, 187.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.360 - 2.054
R-factor	0.188
Rwork	0.186
R-free	0.22280
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.773
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.360	2.218
High resolution limit [Å]	2.054	2.054
Number of reflections	33956	34003
<I/σ(I)>	34.94
Completeness [%]	81.7
Redundancy	1.9
CC(1/2)	1.000	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	PEG 8000,HEPES/ Sodkum hydroxide