9L2K
The crystal structure of SFTSV Gn and SD22 antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.471, 70.346, 177.469 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 41.940 - 2.780 |
| R-factor | 0.3059 |
| Rwork | 0.304 |
| R-free | 0.31220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.780 | 2.800 |
| Rmerge | 0.186 | 1.299 |
| Number of reflections | 19950 | 16920 |
| <I/σ(I)> | 12.322 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.996 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 4 %v/v(+/-)-2-Methy1-2,4-pentanediol, 0.1 M Citric acid pH 3.5, 20 % w/v Polyethylene glycol 1,500 |
