9KSN

Crystal structure of meso-diaminopimelate dehydrogenase from Bacillus thermozeamaize mutant M9 complexed with NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-24
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	156.070, 156.070, 112.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.050 - 2.650
R-factor	0.2028
Rwork	0.201
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zmx
RMSD bond length	0.003
RMSD bond angle	0.718
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.050	2.760
High resolution limit [Å]	2.650	2.650
Number of reflections	40873	4548
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	26.6
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.75	291	20 mM sodium formate; 20 mM ammonium acetate; 20 mM sodium citrate tribasic dihydrate; 20 mM potassium sodium tartrate tetrahydrate; 20 mM sodium oxamate; 100 mM MES/imidazole, pH 6.75; 20% (v/v) ethylene glycol and 10% (w/v) PEG 8000