9KJ9

Anti-CRISPR protein AcrVIA6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	46.680, 85.550, 43.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 2.390
R-factor	0.2206
Rwork	0.212
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.009
RMSD bond angle	0.857
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.479
High resolution limit [Å]	2.390	2.393
Number of reflections	3670	352
<I/σ(I)>	18.08
Completeness [%]	99.5
Redundancy	12.6
CC(1/2)	0.997	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.2 M potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.0