9KBO

Crystal structure of human Shiftless (SFL) containing phosphorylation sites Ser249, Thr250, Thr253 and Ser256

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 2
Unit cell lengths	211.719, 89.411, 82.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.280 - 2.000
R-factor	0.2248
Rwork	0.222
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.847	5.000
High resolution limit [Å]	2.000	2.000
Rmerge	0.175
Number of reflections	106364	16829
<I/σ(I)>	13.4
Completeness [%]	94.4
Redundancy	13.1
CC(1/2)	0.997	0.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	0.2 M Potassium sodium tartrate tetrahydrate, 20 % w/v Polyethylene glycol 3350, pH 7.4