9K8X
Crystal structure of the calcium indicator GCaMP6s-BrUS-145 in calcium-bounded state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-16 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.293, 118.293, 98.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.010 - 2.050 |
| R-factor | 0.21264 |
| Rwork | 0.211 |
| R-free | 0.25065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.603 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.010 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.184 | 1.736 |
| Rmeas | 0.188 | 1.788 |
| Rpim | 0.038 | 0.425 |
| Total number of observations | 1103348 | 59368 |
| Number of reflections | 44474 | 3411 |
| <I/σ(I)> | 17.2 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 24.8 | 17.4 |
| CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.2M Sodium thiocyanate, pH 6.9, 20% PEG 3350 |
