9K7Q

Parkinson disease protein 7 (DJ-1) and Alpha-synuclein (Alpha-syn) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2024-08-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	75.285, 75.274, 75.390
Unit cell angles	89.89, 89.92, 60.04

Refinement procedure

Resolution	7.660 - 4.580
R-factor	0.14866
Rwork	0.145
R-free	0.22247
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.451
Data reduction software	HKL-2000
Data scaling software	XDS
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.610
High resolution limit [Å]	4.530	4.530
Number of reflections	5769	7763
<I/σ(I)>	1.2
Completeness [%]	98.6
Redundancy	3.1
CC(1/2)	0.960	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Sodium iodide, 20% w/v Polyethylene glycol 3,350, pH 7.0