9K34

Human IgG1 Fc fragments, mutant (2CT1.9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	75.988, 101.630, 146.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.990 - 3.000
R-factor	0.2172
Rwork	0.214
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	2.990	8.130	3.000
Rmerge	0.231	0.089	0.779
Rmeas	0.255	0.096	0.920
Rpim	0.105	0.036	0.472
Number of reflections	22816	1300	989
<I/σ(I)>	3.2
Completeness [%]	96.6	99.1	84.2
Redundancy	5.3	6.8	3.1
CC(1/2)	0.958	0.994	0.092

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	50mM HEPES pH 7.0, 10% PEG 3350