9K2Y

Human IgG1 Fc fragments, mutant (2CT1.1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	62.946, 123.325, 150.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.120
R-factor	0.238
Rwork	0.236
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.173	0.096	0.452
Rmeas	0.192	0.102	0.566
Rpim	0.078	0.033	0.333
Total number of observations	92269
Number of reflections	20008	1179	869
<I/σ(I)>	5.2
Completeness [%]	93.1	98.2	82.2
Redundancy	4.6	8.3	2.1
CC(1/2)	0.978	0.995	0.176

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	50mM Sodium phosphate pH 7.0, 16% PEG 3350