9JWV
Structure of WDR5 Y191F in complex with WIN motif containing Kif2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.287, 47.456, 129.523 |
| Unit cell angles | 90.00, 113.23, 90.00 |
Refinement procedure
| Resolution | 58.040 - 1.800 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.020 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.112 | 0.487 |
| Number of reflections | 60375 | 3602 |
| <I/σ(I)> | 6.6 | 2.2 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M HEPES (pH 7.5), 18% PEG 600 |
