9JWD

Crystal structure of RNAs A treated with sodium cyanide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-03-13
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5406
Spacegroup name	P 32 2 1
Unit cell lengths	64.329, 64.329, 64.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.710 - 2.220
R-factor	0.2034
Rwork	0.201
R-free	0.25752
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eic
RMSD bond length	0.008
RMSD bond angle	1.517
Data reduction software	PROTEUM PLUS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.710	2.220
High resolution limit [Å]	2.220	2.220
Rpim		0.521
Number of reflections	7822	683
<I/σ(I)>	1.44
Completeness [%]	99.3
Redundancy	10.5
CC(1/2)	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1M sodium acetate buffer pH 5.5. reservoir solution containing 24-34% (NH4)2SO4, 0.1M sodium acetate buffer pH 5.5, and super-saturated solution of NaCl.