9JWA
Crystal Structure of Lon Bound to a Substrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-05-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 81.050, 81.050, 104.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.670 - 2.290 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.656 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.290 |
| Number of reflections | 30616 | 8901 |
| <I/σ(I)> | 12.51 | |
| Completeness [%] | 94.8 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1ul of 22mg/ml protein complex, 1ul of 0.1M sodium citrate (pH5.0), 20% PEG 2000 (mother), 0.2ul of 30% D(+)-Glucose monohydrate |
