9JJ7

The crystal structure of SARS-CoV-2 NSP5 in complex with eIF4G2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	97.001, 81.980, 51.649
Unit cell angles	90.00, 114.99, 90.00

Refinement procedure

Resolution	43.960 - 1.800
R-factor	0.1951
Rwork	0.194
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.016
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.960	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	66581	33873
<I/σ(I)>	17.24
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M MES pH 5.6, 7% PEG 6000, and 6% dimethyl sulfoxide (DMSO)