9JHQ
Crystal structure of GodF, a post-translational modification enzyme involved in the biosynthesis of goadsporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.340, 69.670, 75.450 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 72.590 - 2.340 |
| R-factor | 0.1863 |
| Rwork | 0.183 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.871 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.600 | 2.430 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.063 | 0.399 |
| Number of reflections | 39491 | 2795 |
| <I/σ(I)> | 6.9 | 2.4 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.9 M sodium acetate, 0.1 M sodium cacodylate |
