9J9W

A broad-spectrum anti-fungal effector dictates bacterial-fungal interkingdom interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 61 2 2
Unit cell lengths	86.930, 86.930, 170.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.730 - 2.600
R-factor	0.2364
Rwork	0.231
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.280	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.085	0.871
Rmeas	0.086	0.883
Rpim	0.014	0.141
Total number of observations	451640	55877
Number of reflections	12420	1468
<I/σ(I)>	34	5.1
Completeness [%]	100.0
Redundancy	36.4	38.1
CC(1/2)	1.000	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277.15	PEG3350, Magnesium chloride, Tris pH8.5