9J6I
Crystal structure of the ABA receptor PYL1 in complex with DBSA compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 |
Unit cell lengths | 126.990, 126.990, 60.195 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.680 - 2.300 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kdj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.035 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.027 | 0.435 |
Number of reflections | 25426 | 2464 |
<I/σ(I)> | 13.35 | 1.82 |
Completeness [%] | 100.0 | |
Redundancy | 6.3 | |
CC(1/2) | 0.999 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 8000, Potassium phosphate dibasic, Glycerol, Ethanol |