9J5G

Autophagy-related proteins of Magnaporthe oryzae-MoAtg8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	71.597, 52.424, 36.443
Unit cell angles	90.00, 114.47, 90.00

Refinement procedure

Resolution	26.210 - 2.500
R-factor	0.2072
Rwork	0.205
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.210	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.087	0.345
Number of reflections	3341	352
<I/σ(I)>	11.77
Completeness [%]	77.0
Redundancy	4.8
CC(1/2)	0.955	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium acetate trihydrate,20 % w/v PEG 3350,10 % v/v Ethylene glycol