9J4U

Structural basis for recognition of SARS-CoV-2 conserved nucleocapside epitopes by dominant T cell receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-09
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	54.757, 148.273, 64.210
Unit cell angles	90.00, 109.15, 90.00

Refinement procedure

Resolution	60.660 - 2.170
R-factor	0.1952
Rwork	0.193
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.700	2.250
High resolution limit [Å]	2.170	2.170
Number of reflections	51116	5086
<I/σ(I)>	16.1
Completeness [%]	99.9
Redundancy	4.8
CC(1/2)	0.999	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M potassium sodium tartrate tetrahydrate, and 20% (w/v) PEG 3350