9J4O
Crystal structure of B. subtilis Leucine tRNA with UAA anticodon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-16 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.190, 37.200, 81.990 |
| Unit cell angles | 90.00, 99.45, 90.00 |
Refinement procedure
| Resolution | 33.796 - 2.500 |
| R-factor | 0.21861 |
| Rwork | 0.216 |
| R-free | 0.26328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.899 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.796 | 2.542 |
| High resolution limit [Å] | 2.499 | 2.499 |
| Rmerge | 0.112 | 1.945 |
| Rmeas | 0.123 | 2.123 |
| Rpim | 0.050 | 0.839 |
| Number of reflections | 8962 | 462 |
| <I/σ(I)> | 9.7 | 1 |
| Completeness [%] | 91.6 | 99.6 |
| Redundancy | 6.1 | 6.3 |
| CC(1/2) | 0.997 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.072 M sodium chloride, 0.036 M sodium cacodylate trihydrate pH 7.0, 27% v/v (+/-)-2-methyl-2,4-pentanediol, 0.0108 M spermine tetrahydrochloride, 0.5% v/v ethyl acetate |
