9J3P

Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-23
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.23984
Spacegroup name	P 21 21 21
Unit cell lengths	74.234, 131.567, 227.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.100
R-factor	0.2187
Rwork	0.217
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	129458	18717
<I/σ(I)>	6.85	0.26
Completeness [%]	100.0	99.9
Redundancy	7	5.7
CC(1/2)	0.999	0.110

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M Sodium sulfate, 20 % w/v PEG 3350