9J23
Structure of a triple-helix region of human Collagen type II from Trautec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-07-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.987, 20.042, 44.985 |
Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
Resolution | 44.300 - 1.750 |
Rwork | 0.241 |
R-free | 0.28790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6f |
RMSD bond length | 0.008 |
RMSD bond angle | 1.489 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.300 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 6457 | 346 |
<I/σ(I)> | 3.5 | |
Completeness [%] | 99.7 | |
Redundancy | 5.6 | |
CC(1/2) | 0.908 | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Index-G12:0.2M MgCl2, 0.1M HEPES pH7.5, 25% PEG3,350 |