9J0Q

Structure of mEos3.2 in the green fluorescent state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	38.675, 70.683, 91.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.080 - 1.340
R-factor	0.1455
Rwork	0.145
R-free	0.16600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ooz
RMSD bond length	0.006
RMSD bond angle	1.436
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.850	1.420
High resolution limit [Å]	1.340	1.340
Number of reflections	56619	8150
<I/σ(I)>	15.81
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Lithium sulfate, 0.1 M MES 6.0, 20 % w/v PEG 4000